Combinatorial assembly and optimisation of designer cellulosomes: a galactomannan case study.

ABSTRACT:

Background

Designer cellulosomes are self-assembled chimeric enzyme complexes that can be used to improve lignocellulosic biomass degradation. They are composed of a synthetic multimodular backbone protein, termed the scaffoldin, and a range of different chimeric docking enzymes that degrade polysaccharides. Over the years, several functional designer cellulosomes have been constructed. Since many parameters influence the efficiency of these multi-enzyme complexes, there is a need to optimise designer cellulosome architecture by testing combinatorial arrangements of docking enzyme and scaffoldin variants. However, the modular cloning procedures are tedious and cumbersome.

Results

VersaTile is a combinatorial DNA assembly method, allowing the rapid construction and thus compa

SUBMITTER: Vanderstraeten J 

PROVIDER: S-EPMC9153192 | biostudies-literature | 2022 May

REPOSITORIES: biostudies-literature