Alternative splicing diversifies the skeletal muscle transcriptome during prolonged spaceflight.

ABSTRACT:

Background

As the interest in manned spaceflight increases, so does the requirement to understand the transcriptomic mechanisms that underlay the detrimental physiological adaptations of skeletal muscle to microgravity. While microgravity-induced differential gene expression (DGE) has been extensively investigated, the contribution of differential alternative splicing (DAS) to the plasticity and functional status of the skeletal muscle transcriptome has not been studied in an animal model. Therefore, by evaluating both DGE and DAS across spaceflight, we set out to provide the first comprehensive characterization of the transcriptomic landscape of skeletal muscle during exposure to microgravity.

Methods

RNA-sequencing, immunohistochemistry, and morphological analyses were con

SUBMITTER: Henrich M 

PROVIDER: S-EPMC9153194 | biostudies-literature | 2022 May

REPOSITORIES: biostudies-literature