Extracellular miR-6723-5p could serve as a biomarker of limbal epithelial stem/progenitor cell population.

ABSTRACT:

Background

Dysfunction or loss of limbal stem cells can result in limbal stem cell deficiency (LSCD), a disease that cause corneal opacity, pain, and loss of vision. Cultivated limbal epithelial transplantation (CLET) can be used to restore stem cell niche homeostasis and replenish the progenitor pool. Transplantation has been reported with high success rate, but there is an unmet need of prognostic markers that correlate with clinical outcomes. To date, the progenitor content in the graft is the only parameter that has been retrospectively linked to success.

Methods

In this study, we investigate extracellular micro RNAs (miRNAs) associated with stem/progenitor cells in cultivated limbal epithelial cells (cLECs). Using micro RNA sequencing and linear regression modelling, we

SUBMITTER: Ruiz M 

PROVIDER: S-EPMC9153202 | biostudies-literature | 2022 May

REPOSITORIES: biostudies-literature