Single-cell profiling of transcriptome and histone modifications with EpiDamID.

ABSTRACT: Recent advances in single-cell sequencing technologies have enabled simultaneous measurement of multiple cellular modalities, but the combined detection of histone post-translational modifications and transcription at single-cell resolution has remained limited. Here, we introduce EpiDamID, an experimental approach to target a diverse set of chromatin types by leveraging the binding specificities of single-chain variable fragment antibodies, engineered chromatin reader domains, and endogenous chromatin-binding proteins. Using these, we render the DamID technology compatible with the genome-wide identification of histone post-translational modifications. Importantly, this includes the possibility to jointly measure chromatin marks and transcription at the single-cell level. We use EpiDamID

SUBMITTER: Rang FJ 

PROVIDER: S-EPMC9153956 | biostudies-literature | 2022 May

REPOSITORIES: biostudies-literature