Underground metabolism as a rich reservoir for pathway engineering.

ABSTRACT:

Motivation

Bioproduction of value-added compounds is frequently achieved by utilizing enzymes from other species. However, expression of such heterologous enzymes can be detrimental due to unexpected interactions within the host cell. Recently, an alternative strategy emerged, which relies on recruiting side activities of host enzymes to establish new biosynthetic pathways. Although such low-level 'underground' enzyme activities are prevalent, it remains poorly explored whether they may serve as an important reservoir for pathway engineering.

Results

Here, we use genome-scale modeling to estimate the theoretical potential of underground reactions for engineering novel biosynthetic pathways in Escherichia coli. We found that biochemical reactions contributed by underground en

SUBMITTER: Kovacs SC 

PROVIDER: S-EPMC9154287 | biostudies-literature | 2022 May

REPOSITORIES: biostudies-literature