Cardamonin suppressed the migration, invasion, epithelial mesenchymal transition (EMT) and lung metastasis of colorectal cancer cells by down-regulating ADRB2 expression.

ABSTRACT:

Context

Cardamonin (CDN) can suppress cell growth in colorectal cancer (CRC), a common digestive malignancy.

Objective

We explored the effect and mechanism of CDN on metastatic CRC.

Materials and methods

Two cell lines (HT29 and HCT116) were initially treated with CDN at different concentrations (5, 10 and 20 μmol/L) or 50 μmol/L propranolol (positive control) for 24 or 48 h. Then, the two cell lines were separately transfected with siADRB2 and ADRB2 overexpression plasmids, and further treated with 10 μmol/L CDN for 24 h. The cell viability, migration and invasion were determined by cell counting kit-8 (CCK-8), wound healing and transwell assays, respectively. The levels of ADRB2, matrix metalloprotease (MMP)-2, MMP-9, E-cadherin and N-cadherin were measured by Weste

SUBMITTER: Lu T 

PROVIDER: S-EPMC9154753 | biostudies-literature | 2022 Dec

REPOSITORIES: biostudies-literature