Effects of obesity on reparative function of human adipose tissue-derived mesenchymal stem cells on ischemic murine kidneys.

ABSTRACT:

Introduction

Obesity is a health burden that impairs cellular processes. Mesenchymal stem/stromal cells (MSCs) are endowed with reparative properties and can ameliorate renal injury. Obesity impairs human MSC function in-vitro, but its effect on their in-vivo reparative potency remains unknown.

Subjects and methods

Abdominal adipose tissue-derived MSC were harvested from patients without ('lean') or with obesity ('obese') (body mass index <30 or ≥30 kg/m², respectively) during kidney donation or bariatric surgery, respectively. MSC (5 × 10⁵/200 µL) or vehicle were then injected into 129S1 mice 2 weeks after renal artery stenosis (RAS) or sham surgery (n = 8/group). Two weeks later, mice underwent magnetic resonance imaging to assess renal perfusion and

SUBMITTER: Klomjit N 

PROVIDER: S-EPMC9156526 | biostudies-literature | 2022 Jun

REPOSITORIES: biostudies-literature