Sustained delivery of focal ischemia coupled to real-time neurochemical sensing in brain slices.

ABSTRACT: Local stimulation of tissue can occur naturally in events like immune-mediated inflammation and focal ischemic injuries in brain and is confined to specific regions within tissue, occurring on various timescales. Making chemical measurements at the exact site of stimulation with current technologies is difficult yet important for understanding tissue response. We have developed a microfluidic device capable of local stimulation of brain slices with minimal lateral spread over time and submillimeter, tunable spatial resolution. This device is compatible with electrochemical measurements to monitor signaling at the site of stimulation over time. The PDMS-based device is three layers and contains a culture well, channel layer, and exit port layer for the channels. Channels with exit ports str

SUBMITTER: Cryan MT 

PROVIDER: S-EPMC9156565 | biostudies-literature | 2022 May

REPOSITORIES: biostudies-literature