Physical optimization of cell proliferation and differentiation using spinner flask and microcarriers.

ABSTRACT: The traditional breeding industry has been increasingly saturated and caused environmental pollution, disease transmission, excessive resource use, and methane emission; however, it still cannot meet the needs of the growing population. To explore other alternatives, researchers focused on cell agriculture and cell-based meat, especially large-scale cell culture. As a prerequisite for production, large-scale culture technology has become an important bottleneck restricting cell-based meat industrialization. In this study, the single-factor variable method was adopted to examine the influence of Cytodex1 microcarrier pretreatment, spinner flask reaction vessel, cell culture medium, serum and cell incubation, and other influencing factors on large-scale cell cultures to identify the optimiza

SUBMITTER: Yang F 

PROVIDER: S-EPMC9156609 | biostudies-literature | 2022 May

REPOSITORIES: biostudies-literature