Structural variant-based pangenome construction has low sensitivity to variability of haplotype-resolved bovine assemblies.

ABSTRACT: Advantages of pangenomes over linear reference assemblies for genome research have recently been established. However, potential effects of sequence platform and assembly approach, or of combining assemblies created by different approaches, on pangenome construction have not been investigated. Here we generate haplotype-resolved assemblies from the offspring of three bovine trios representing increasing levels of heterozygosity that each demonstrate a substantial improvement in contiguity, completeness, and accuracy over the current Bos taurus reference genome. Diploid coverage as low as 20x for HiFi or 60x for ONT is sufficient to produce two haplotype-resolved assemblies meeting standards set by the Vertebrate Genomes Project. Structural variant-based pangenomes created from the haplotyp

SUBMITTER: Leonard AS 

PROVIDER: S-EPMC9156671 | biostudies-literature | 2022 May

REPOSITORIES: biostudies-literature