Dataset Information

Functional and phylogenetic analysis of TetX variants to design a new classification system.

ABSTRACT: Recently, many TetX variants such as Tet(X3~14) were reported to confer resistance to tigecycline which is a last-resort antibiotic used to treat infections caused by multidrug-resistant bacteria. In this study, we identified essential residues including 329, 339, 340, 350, and 351 in TetX variants that mediated the evolution of the tigecycline-inactive Tet(X2) enzyme to the active forms of Tet(X3) and Tet(X4). Based on their amino acid sequences and functional features, we classified TetX variants into TetX-A class, TetX-B class and TetX-C class. We further found that TetX-A class variants originated from Bacteroidetes, with some variants further evolving to TetX-C class and acquired by Enterobacteriaceae. On the other hand, our data showed that some variants genes belonging to TetX-A class evolved directly to TetX-B class, which was further transmitted to Acinetobacter spp. This new classification system may facilitate better clinical management of patients infected by TetX-producing strains.

SUBMITTER: Cheng Q

PROVIDER: S-EPMC9156754 | biostudies-literature | 2022 May

REPOSITORIES: biostudies-literature

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Functional and phylogenetic analysis of TetX variants to design a new classification system.

Cheng Qipeng Q Cheung Yanchu Y Liu Chenyu C Chan Edward Wai Chi EWC Wong Kwok Yin KY Zhang Rong R Chen Sheng S Chen Sheng S

Communications biology 20220531 1

Recently, many TetX variants such as Tet(X3~14) were reported to confer resistance to tigecycline which is a last-resort antibiotic used to treat infections caused by multidrug-resistant bacteria. In this study, we identified essential residues including 329, 339, 340, 350, and 351 in TetX variants that mediated the evolution of the tigecycline-inactive Tet(X2) enzyme to the active forms of Tet(X3) and Tet(X4). Based on their amino acid sequences and functional features, we classified TetX varia ...[more]

PMID: 35641548

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Dataset Information

Functional and phylogenetic analysis of TetX variants to design a new classification system.

Publications

Functional and phylogenetic analysis of TetX variants to design a new classification system.

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