Salt stress alters membrane lipid content and lipid biosynthesis pathways in the plasma membrane and tonoplast.

ABSTRACT: Plant cell membranes are the sites of sensing and initiation of rapid responses to changing environmental factors including salinity stress. Understanding the mechanisms involved in membrane remodeling is important for studying salt tolerance in plants. This task remains challenging in complex tissue due to suboptimal subcellular membrane isolation techniques. Here, we capitalized on the use of a surface charge-based separation method, free flow electrophoresis, to isolate the tonoplast (TP) and plasma membrane (PM) from leaf tissue of the halophyte ice plant (Mesembryanthemum crystallinum L.). Results demonstrated a membrane-specific lipidomic remodeling in this plant under salt conditions, including an increased proportion of bilayer forming lipid phosphatidylcholine in the TP and an inc

SUBMITTER: Guo Q 

PROVIDER: S-EPMC9157097 | biostudies-literature | 2022 Jun

REPOSITORIES: biostudies-literature