Overdominant and partially dominant mutations drive clonal adaptation in diploid Saccharomyces cerevisiae.

ABSTRACT: Identification of adaptive targets in experimental evolution typically relies on extensive replication and genetic reconstruction. An alternative approach is to directly assay all mutations in an evolved clone by generating pools of segregants that contain random combinations of evolved mutations. Here, we apply this method to 6 Saccharomyces cerevisiae clones isolated from 4 diploid populations that were clonally evolved for 2,000 generations in rich glucose medium. Each clone contains 17-26 mutations relative to the ancestor. We derived intermediate genotypes between the founder and the evolved clones by bulk mating sporulated cultures of the evolved clones to a barcoded haploid version of the ancestor. We competed the resulting barcoded diploids en masse and quantified fitness in the ex

SUBMITTER: Aggeli D 

PROVIDER: S-EPMC9157133 | biostudies-literature | 2022 May

REPOSITORIES: biostudies-literature