Highly efficient site-specific integration of DNA fragments into the honeybee genome using CRISPR/Cas9.

ABSTRACT: Functional genetic studies in honeybees have been limited to transposon mediated transformation and site directed mutagenesis tools. However, site- and sequence-specific manipulations that insert DNA fragments or replace sequences at specific target sites are lacking. Such tools would enable the tagging of proteins, the expression of reporters and site-specific amino acid changes, which are all gold standard manipulations for physiological, organismal, and genetic studies. However, such manipulations must be very efficient in honeybees since screening and crossing procedures are laborious due to their social organization. Here, we report an accurate and remarkably efficient site-specific integration of DNA-sequences into the honeybee genome using clustered regularly interspaced short palin

SUBMITTER: Wagner A 

PROVIDER: S-EPMC9157169 | biostudies-literature | 2022 May

REPOSITORIES: biostudies-literature