Evaluation and optimization of analytical procedure and sample preparation for polar Streptomyces albus J1074 metabolome profiling.

ABSTRACT: Metabolomics is an essential discipline in omics technology that promotes research on the biology of microbial systems. Streptomyces albus J1074 is a model organism used in fundamental research and industrial microbiology. Nevertheless, a comprehensive and standardized method for analyzing the metabolome of S. albus J1074 is yet to be developed. Thus, we comprehensively evaluated and optimized the analytical procedure and sample preparation for profiling polar metabolites using hydrophilic interaction liquid chromatography (HILIC) coupled with high-resolution mass spectrometry (HRMS). We systematically examined the HILIC columns, quenching solutions, sample-to-quenching ratios, and extraction methods. Then, the optimal protocol was used to investigate the dynamic intracellula

SUBMITTER: Fu S 

PROVIDER: S-EPMC9157217 | biostudies-literature | 2022 Sep

REPOSITORIES: biostudies-literature