Enhanced cobalamin biosynthesis in Ensifer adhaerens by regulation of key genes with gradient promoters.

ABSTRACT: Cobalamin is an essential human vitamin widely used in the pharmaceutical, food, and feed additive industries and currently produced by bacteria or archaea. Ensifer adhaerens HY-1 is an industrial strain that also produces cobalamin. However production outputs are poor and the specific synthesis pathways require characterization. In this study, the whole genome sequence of E. adhaerens HY-1 was generated and annotated, and genes associated with cobalamin biosynthesis were identified. Then, three genes, CobSV, CobQ, and CobW were identified as the most efficient ones for enhancing cobalamin synthesis. By transcriptome sequencing of E. adhaerens HY-1 cells at different growth stages, 65 endogenous promoters with different gradient strengths were iden

SUBMITTER: Xu S 

PROVIDER: S-EPMC9157374 | biostudies-literature | 2022 Sep

REPOSITORIES: biostudies-literature