Characterizing the Piezosphere: The Effects of Decompression on Microbial Growth Dynamics.

ABSTRACT: The extent to which the full diversity of the subsurface microbiome can be captured via cultivation is likely hindered by the inevitable loss of cellular viability from decompression during sampling, enrichment, and isolation. Furthermore, the pressure tolerance of previously isolated strains that span surface and subsurface ecosystems can shed light into microbial activity and pressure adaptation in these transition zones. However, assessments of the effects of elevated pressure on the physiology of piezotolerant and piezosensitive species may be biased by high-pressure enrichment techniques. Here, we compared two high-pressure cultivation techniques-one that requires decompression of the whole cultures during sampling and one that employs the previously described isobaric PUSH dev

SUBMITTER: Cario A 

PROVIDER: S-EPMC9157427 | biostudies-literature | 2022

REPOSITORIES: biostudies-literature