Modeling the effect of rRNA-mRNA interactions and mRNA folding on mRNA translation in chloroplasts.

ABSTRACT: The process of translation initiation in prokaryotes is mediated by the hybridization of the 16S rRNA of the small ribosomal subunit with the mRNA in a short region called the ribosomal binding site. However, translation initiation in chloroplasts, which have evolved from an ancestral bacterium, is not well understood. Some studies suggest that in many cases it differs from translation initiation in bacteria and involves various novel interactions of the mRNA structures with intracellular factors; however currently, there is no generic quantitative model related to these aspects in chloroplasts. We developed a novel computational pipeline and models that can be used for understanding and modeling translation regulation in chloroplasts. We demonstrate that local folding and co-folding energ

SUBMITTER: Carmel Ezra S 

PROVIDER: S-EPMC9157439 | biostudies-literature | 2022

REPOSITORIES: biostudies-literature