Overcoming universal restrictions on metal selectivity by protein design.

ABSTRACT: Selective metal coordination is central to the functions of metalloproteins:^1,2 each metalloprotein must pair with its cognate metallocofactor to fulfil its biological role³. However, achieving metal selectivity solely through a three-dimensional protein structure is a great challenge, because there is a limited set of metal-coordinating amino acid functionalities and proteins are inherently flexible, which impedes steric selection of metals^3,4. Metal-binding affinities of natural proteins are primarily dictated by the electronic properties of metal ions and follow the Irving-Williams series⁵ (Mn²⁺ < Fe²⁺ < Co²⁺ < Ni²⁺ < Cu²⁺ > Zn²⁺) with few exceptions^6,7. Accordingly,

SUBMITTER: Choi TS 

PROVIDER: S-EPMC9157509 | biostudies-literature | 2022 Mar

REPOSITORIES: biostudies-literature