Characterization of Novel Human Immortalized Thyroid Follicular Epithelial Cell Lines.

ABSTRACT:

Introduction

Investigation of normal human thyroid function using in vitro culture systems is dependent on cells that recapitulate physiology of differentiated thyrocytes. Primary thyrocytes retain features of the native organ but have limited lifespan in culture. Immortalized thyrocytes offer an alternative if challenges maintaining phenotypic stability can be overcome to retain functional features of primary cells.

Materials and methods

CI-SCREEN immortalization technology was applied to normal human thyroid tissue to generate four cell line variants. The lines were characterized for transgene integration, biomarker expression, genomic stability, and proliferation rates. Thyroid Stimulating Hormone (TSH)-dependent morphology, thyroglobulin production, thyroxine horm

SUBMITTER: Hopperstad K 

PROVIDER: S-EPMC9157743 | biostudies-literature | 2021 Jun

REPOSITORIES: biostudies-literature