Depletion of Toxoplasma adenine nucleotide translocator leads to defects in mitochondrial morphology.

ABSTRACT:

Background

Adenine nucleotide translocase (ANT) is a protein that catalyzes the exchange of ADP/ATP across the inner mitochondrial membrane. Beyond this, ANT is closely associated with cell death pathways and mitochondrial dysfunction. It is a potential therapeutic target for many diseases. The function of the ANT in Toxoplasma gondii is poorly understood.

Methods

The CRISPR/CAS9 gene editing tool was used to identify and study the function of the ANT protein in T. gondii. We constructed T. gondii ANT transgenic parasite lines, including endogenous tag strain, knockout strain and gene complement strain, to clarify the function and location of TgANT. Mitochondrial morphology was observed by immunofluorescence and transmission electron microscopy.

Results

Toxoplasma gon

SUBMITTER: Wu Y 

PROVIDER: S-EPMC9158195 | biostudies-literature | 2022 May

REPOSITORIES: biostudies-literature