Leveraging transcriptome and epigenome landscapes to infer regulatory networks during the onset of sexual maturation.

ABSTRACT:

Background

Despite sexual development being ubiquitous to vertebrates, the molecular mechanisms underpinning this fundamental transition remain largely undocumented in many organisms. We designed a time course experiment that successfully sampled the period when Atlantic salmon commence their trajectory towards sexual maturation.

Results

Through deep RNA sequencing, we discovered key genes and pathways associated with maturation in the pituitary-ovarian axis. Analyzing DNA methylomes revealed a bias towards hypermethylation in ovary that implicated maturation-related genes. Co-analysis of DNA methylome and gene expression changes revealed chromatin remodeling genes and key transcription factors were both significantly hypermethylated and upregulated in the ovary during the o

SUBMITTER: Mohamed AR 

PROVIDER: S-EPMC9158274 | biostudies-literature | 2022 Jun

REPOSITORIES: biostudies-literature