Dataset Information

Leveraging transcriptome and epigenome landscapes to infer regulatory networks during the onset of sexual maturation.

ABSTRACT:

Background

Despite sexual development being ubiquitous to vertebrates, the molecular mechanisms underpinning this fundamental transition remain largely undocumented in many organisms. We designed a time course experiment that successfully sampled the period when Atlantic salmon commence their trajectory towards sexual maturation.

Results

Through deep RNA sequencing, we discovered key genes and pathways associated with maturation in the pituitary-ovarian axis. Analyzing DNA methylomes revealed a bias towards hypermethylation in ovary that implicated maturation-related genes. Co-analysis of DNA methylome and gene expression changes revealed chromatin remodeling genes and key transcription factors were both significantly hypermethylated and upregulated in the ovary during the onset of maturation. We also observed changes in chromatin state landscapes that were strongly correlated with fundamental remodeling of gene expression in liver. Finally, a multiomic integrated analysis revealed regulatory networks and identified hub genes including TRIM25 gene (encoding the estrogen-responsive finger protein) as a putative key regulator in the pituitary that underwent a 60-fold change in connectivity during the transition to maturation.

Conclusion

The study successfully documented transcriptome and epigenome changes that involved key genes and pathways acting in the pituitary - ovarian axis. Using a Systems Biology approach, we identified hub genes and their associated networks deemed crucial for onset of maturation. The results provide a comprehensive view of the spatiotemporal changes involved in a complex trait and opens the door to future efforts aiming to manipulate puberty in an economically important aquaculture species.

SUBMITTER: Mohamed AR

PROVIDER: S-EPMC9158274 | biostudies-literature | 2022 Jun

REPOSITORIES: biostudies-literature

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Publications

Leveraging transcriptome and epigenome landscapes to infer regulatory networks during the onset of sexual maturation.

Mohamed Amin R AR Naval-Sanchez Marina M Menzies Moira M Evans Bradley B King Harry H Reverter Antonio A Kijas James W JW

BMC genomics 20220601 1

<h4>Background</h4>Despite sexual development being ubiquitous to vertebrates, the molecular mechanisms underpinning this fundamental transition remain largely undocumented in many organisms. We designed a time course experiment that successfully sampled the period when Atlantic salmon commence their trajectory towards sexual maturation.<h4>Results</h4>Through deep RNA sequencing, we discovered key genes and pathways associated with maturation in the pituitary-ovarian axis. Analyzing DNA methylo ...[more]

PMID: 35650521

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Dataset Information

Leveraging transcriptome and epigenome landscapes to infer regulatory networks during the onset of sexual maturation.

Background

Results

Conclusion

Publications

Leveraging transcriptome and epigenome landscapes to infer regulatory networks during the onset of sexual maturation.

Similar Datasets

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