Natural antisense RNA Foxk1-AS promotes myogenic differentiation by inhibiting Foxk1 activity.

ABSTRACT:

Background

Natural antisense RNAs are RNA molecules that are transcribed from the opposite strand of either protein-coding or non-protein coding genes and have the ability to regulate the expression of their sense gene or several related genes. However, the roles of natural antisense RNAs in the maintenance and myogenesis of muscle stem cells remain largely unexamined.

Methods

We analysed myoblast differentiation and regeneration by overexpression and knockdown of Foxk1-AS using lentivirus and adeno-associated virus infection in C2C12 cells and damaged muscle tissues. Muscle injury was induced by BaCl₂ and the regeneration and repair of damaged muscle tissues was assessed by haematoxylin-eosin staining and quantitative real-time PCR. The expression of myogenic dif

SUBMITTER: Li C 

PROVIDER: S-EPMC9158385 | biostudies-literature | 2022 May

REPOSITORIES: biostudies-literature