Lipidic cubic phase serial femtosecond crystallography structure of a photosynthetic reaction centre.

ABSTRACT: Serial crystallography is a rapidly growing method that can yield structural insights from microcrystals that were previously considered to be too small to be useful in conventional X-ray crystallography. Here, conditions for growing microcrystals of the photosynthetic reaction centre of Blastochloris viridis within a lipidic cubic phase (LCP) crystallization matrix that employ a seeding protocol utilizing detergent-grown crystals with a different crystal packing are described. LCP microcrystals diffracted to 2.25 Å resolution when exposed to XFEL radiation, which is an improvement of 0.15 Å over previous microcrystal forms. Ubiquinone was incorporated into the LCP crystallization media and the resulting electron density within the mobile Q_B pocket is comparable to that of other

SUBMITTER: Bath P 

PROVIDER: S-EPMC9159286 | biostudies-literature | 2022 Jun

REPOSITORIES: biostudies-literature