Combining Cellular Immunology With RNAseq to Identify Novel Chlamydia T-Cell Subset Signatures.

ABSTRACT: Chlamydia trachomatis serovars A-L cause important diseases of the eyes and reproductive tract by infecting epithelium lining those organs. A major hurdle for vaccine trials is finding a surrogate biomarker for protective immunity. Investigational data argues for T-cell biomarker(s) reflecting mucosal adaption, cytokine polarization, B-cell help, antibacterial effector mechanisms, or some combination thereof. A human investigation and 2 mouse studies link IL-13 to protection from infection/immunopathology. We performed RNAseq on T cells resident in spleens and genital tracts of naturally immune mice. CD4 signatures were consistent with helper function that differed by site including a genital tract-specific Fgl2 signal. The genital tract CD8 signature featured IL-10 and promotion of healin

SUBMITTER: Johnson RM 

PROVIDER: S-EPMC9159333 | biostudies-literature | 2022 Jun

REPOSITORIES: biostudies-literature