Small molecules released from islets of Langerhans determined by liquid chromatography - mass spectrometry.

ABSTRACT: Islets of Langerhans are the endocrine tissue within the pancreas that secrete hormones for maintenance of blood glucose homeostasis. A variety of small molecules including classical neurotransmitters are also released from islets. While the roles of most of these small molecules are unknown, some have been hypothesized to play a critical role in islet physiology. To better understand their role on islet function, a liquid chromatography-tandem mass spectrometry (LC-MS/MS) method was developed to separate and quantify 39 small molecules released from islets. Benzoyl chloride derivatization of analyte molecules was used to impart retention and facilitate electrospray ionization efficiency. Separation was achieved on a 2.1 × 150 mm column packed with 2.7 μm core-shell C₁₈ particle

SUBMITTER: Ogunkunle EO 

PROVIDER: S-EPMC9159447 | biostudies-literature | 2022 Jun

REPOSITORIES: biostudies-literature