Butyric Acid Ameliorates Myocardial Fibrosis by Regulating M1/M2 Polarization of Macrophages and Promoting Recovery of Mitochondrial Function.

ABSTRACT:

Background

We aimed to investigate the effect and mechanism of butyric acid on rat myocardial fibrosis (MF).

Methods

16S rRNA sequencing was used to analyze the gut microbiota characteristics of the Sham group and MF group. HPLC was applied to measure butyric acid in the feces and serum. In vitro, rat macrophages RMa-bm were stimulated with LPS and IL-4, respectively, and then butyrate was added to study the influences of butyrate on M1/M2 polarization and mitochondrial function of rat macrophages. The rat macrophages and rat myocardial fibroblasts were co-cultured to explore the effect of butyrate on rat myocardial fibroblasts. In addition, MF rats were fed with butyric acid diet.

Results

Compared with the Sham group, collagen deposition in the MF group was in

SUBMITTER: Li X 

PROVIDER: S-EPMC9159497 | biostudies-literature | 2022

REPOSITORIES: biostudies-literature