Methanotrophy Alleviates Nitrogen Constraint of Carbon Turnover by Rice Root-Associated Microbiomes.

ABSTRACT: The bioavailability of nitrogen constrains primary productivity, and ecosystem stoichiometry implies stimulation of N₂ fixation in association with carbon sequestration in hotspots such as paddy soils. In this study, we show that N₂ fixation was triggered by methane oxidation and the methanotrophs serve as microbial engines driving the turnover of carbon and nitrogen in rice roots. ¹⁵N₂-stable isotope probing showed that N₂-fixing activity was stimulated 160-fold by CH₄ oxidation from 0.27 to 43.3 μmol N g^-1 dry weight root biomass, and approximately 42.5% of the fixed N existed in the form of ¹⁵N-NH₄ ⁺ through microbial mineralization. Nitrate amendment almost completely abolished

SUBMITTER: Cao W 

PROVIDER: S-EPMC9159908 | biostudies-literature | 2022

REPOSITORIES: biostudies-literature