Cell radiolabeling with acoustophoresis cell washing.

ABSTRACT: Labeling immune cells with zirconium-89 (⁸⁹Zr)-oxine has become a viable method to track cells in vivo by PET in various pre-clinical animal models and in clinical applications. Currently, ⁸⁹Zr-oxine cell labeling is performed manually, which requires a highly trained specialist and is prone to human error. As the first phase in developing a fully automated radiolabeling system to address this problem, we assess the use of acoustophoresis cell washing to replace the centrifugal cell washing used in the current ⁸⁹Zr-oxine cell radiolabeling procedure. To accomplish this, a cell radiolabeling procedure was developed in which two steps requiring a centrifuge to wash cells were replaced using acoustophoresis cell washing methods. The process was tested using mu

SUBMITTER: Adler SS 

PROVIDER: S-EPMC9160075 | biostudies-literature | 2022 Jun

REPOSITORIES: biostudies-literature