Transport of intensity diffraction tomography with non-interferometric synthetic aperture for three-dimensional label-free microscopy.

ABSTRACT: We present a new label-free three-dimensional (3D) microscopy technique, termed transport of intensity diffraction tomography with non-interferometric synthetic aperture (TIDT-NSA). Without resorting to interferometric detection, TIDT-NSA retrieves the 3D refractive index (RI) distribution of biological specimens from 3D intensity-only measurements at various illumination angles, allowing incoherent-diffraction-limited quantitative 3D phase-contrast imaging. The unique combination of z-scanning the sample with illumination angle diversity in TIDT-NSA provides strong defocus phase contrast and better optical sectioning capabilities suitable for high-resolution tomography of thick biological samples. Based on an off-the-shelf bright-field microscope with a programmable light-emitting-diode (

SUBMITTER: Li J 

PROVIDER: S-EPMC9160286 | biostudies-literature | 2022 Jun

REPOSITORIES: biostudies-literature