Validation of an Accurate Automated Multiplex Immunofluorescence Method for Immuno-Profiling Melanoma.

ABSTRACT: Multiplex immunofluorescence staining enables the simultaneous detection of multiple immune markers in a single tissue section, and is a useful tool for the identification of specific cell populations within the tumour microenvironment. However, this technology has rarely been validated against standard clinical immunohistology, which is a barrier for its integration into clinical practice. This study sought to validate and investigate the accuracy, precision and reproducibility of a multiplex immunofluorescence compared with immunohistochemistry (IHC), including tissue staining, imaging and analysis, in characterising the expression of immune and melanoma markers in both the tumour and its microenvironment. Traditional chromogenic IHC, single-plex immunofluorescence and multiplex immunofl

SUBMITTER: Yaseen Z 

PROVIDER: S-EPMC9160303 | biostudies-literature | 2022

REPOSITORIES: biostudies-literature