HuR Affects the Radiosensitivity of Esophageal Cancer by Regulating the EMT-Related Protein Snail.

ABSTRACT:

Purpose

We previously found that Hu antigen R (HuR) can regulate the proliferation and metastasis of esophageal cancer cells. This study aims to explore the effects of HuR on the radiosensitivity of esophageal cancer.

Materials and method

Analyses of CCK-8, colony formation assay, Western blot, immunofluorescence, flow cytometry, reactive oxygen species (ROS), and mitochondrial membrane potential were conducted to characterize the esophageal cancer cells. Nude mouse models were used to detect the effects of HuR in a combination of X-ray treatment on the subcutaneous xenografts of esophageal cancer. In addition, a luciferase assay was used to detect the direct interaction of HuR with Snail mRNA 3'-UTR.

Results

The down-regulation of HuR combined with X-ray can signific

SUBMITTER: Hu Y 

PROVIDER: S-EPMC9160430 | biostudies-literature | 2022

REPOSITORIES: biostudies-literature