Normalizing the Optical Signal Enables Robust Assays with Lateral Flow Biosensors.

ABSTRACT: Lateral flow assays (LFAs) are widely adopted for fast, on-site molecular diagnostics. Obtaining high-precision assay results, however, remains challenging and often requires a dedicated optical setup to control the imaging environment. Here, we describe quick light normalization exam (qLiNE) that transforms ubiquitous smartphones into a robust LFA reader. qLiNE used a reference card, printed with geometric patterns and color standards, for real-time optical calibration: a photo of an LFA test strip was taken along with the card, and the image was processed using a smartphone app to correct shape distortion, illumination brightness, and color imbalances. This approach yielded consistent optical signal, enabling quantitative molecular analyses under different illumination conditions. We ada

SUBMITTER: Park JH 

PROVIDER: S-EPMC9161384 | biostudies-literature | 2022 May

REPOSITORIES: biostudies-literature