5'isomiR-183-5p|+2 elicits tumor suppressor activity in a negative feedback loop with E2F1.

ABSTRACT:

Background

MicroRNAs (miRNAs) and isomiRs play important roles in tumorigenesis as essential regulators of gene expression. 5'isomiRs exhibit a shifted seed sequence compared to the canonical miRNA, resulting in different target spectra and thereby extending the phenotypic impact of the respective common pre-miRNA. However, for most miRNAs, expression and function of 5'isomiRs have not been studied in detail yet. Therefore, this study aims to investigate the functions of miRNAs and their 5'isomiRs.

Methods

The expression of 5'isomiRs was assessed in The Cancer Genome Atlas (TCGA) breast cancer patient dataset. Phenotypic effects of miR-183 overexpression in triple-negative breast cancer (TNBC) cell lines were investigated in vitro and in vivo by quantifying migration, prolif

SUBMITTER: Li X 

PROVIDER: S-EPMC9161486 | biostudies-literature | 2022 Jun

REPOSITORIES: biostudies-literature