Bio-informatic analysis of CRISPR protospacer adjacent motifs (PAMs) in T4 genome.

ABSTRACT:

Background

The existence of protospacer adjacent motifs (PAMs) sequences in bacteriophage genome is critical for the recognition and function of the clustered regularly interspaced short palindromic repeats-Cas (CRISPR-Cas) machinery system. We further elucidate the significance of PAMs and their function, particularly as a part of transcriptional regulatory regions in T4 bacteriophages.

Methods

A scripting language was used to analyze a sequence of T4 phage genome, and a list of few selected PAMs. Mann-Whitney Wilcoxon (MWW) test was used to compare the sequence hits for the PAMs versus the hits of all the possible sequences of equal lengths.

Results

The results of MWW test show that certain PAMs such as: 'NGG' and 'TATA' are preferably located at the core of phage p

SUBMITTER: Rawashdeh O 

PROVIDER: S-EPMC9161530 | biostudies-literature | 2022 Jun

REPOSITORIES: biostudies-literature