PyZebrascope: An Open-Source Platform for Brain-Wide Neural Activity Imaging in Zebrafish

ABSTRACT: Understanding how neurons interact across the brain to control animal behaviors is one of the central goals in neuroscience. Recent developments in fluorescent microscopy and genetically-encoded calcium indicators led to the establishment of whole-brain imaging methods in zebrafish, which record neural activity across a brain-wide volume with single-cell resolution. Pioneering studies of whole-brain imaging used custom light-sheet microscopes, and their operation relied on commercially developed and maintained software not available globally. Hence it has been challenging to disseminate and develop the technology in the research community. Here, we present PyZebrascope, an open-source Python platform designed for neural activity imaging in zebrafish using light-sheet microscopy. PyZebrasco

SUBMITTER: Barbara R 

PROVIDER: S-EPMC9161555 | biostudies-literature | 2022 Jan

REPOSITORIES: biostudies-literature