Genome-wide DNA methylation reveals potential epigenetic mechanism of age-dependent viral susceptibility in grass carp.

ABSTRACT:

Background

Grass carp are an important farmed fish in China that are infected by many pathogens, especially grass carp reovirus (GCRV). Notably, grass carp showed age-dependent susceptibility to GCRV; that is, grass carp not older than one year were sensitive to GCRV, while those over three years old were resistant to this virus. However, the underlying mechanism remains unclear. Herein, whole genome-wide DNA methylation and gene expression variations between susceptible five-month-old (FMO) and resistant three-year-old (TYO) grass carp were investigated aiming to uncover potential epigenetic mechanisms.

Results

Colorimetric quantification revealed that the global methylation level in TYO fish was higher than that in FMO fish. Whole-genome bisulfite sequencing (WGBS) of the

SUBMITTER: He L 

PROVIDER: S-EPMC9161582 | biostudies-literature | 2022 Jun

REPOSITORIES: biostudies-literature