Overlapping promoter library designed for rational heterogenous expression in Cordyceps militaris.

ABSTRACT:

Background

Cordyceps militaris, a kind of edible and medicinal fungus widely accepted in East Asia, has attracted much attention as a potential cell factory for producing adenosine analogs. Despite the rapid development in gene editing techniques and genome modeling, the diversity of DNA elements in C. militaris was too short to achieve rational heterogeneous expression for metabolic engineering studies.

Results

In this study, P_trpC, a kind of promoter with a relatively appropriate expression level and small size, was selected as a monomer for promoter library construction. Through in vitro BioBricks assembly, 9 overlapping P_trpC promoters with different copy numbers as well as reporter gene gfp were connected and subsequently integrated into the genome

SUBMITTER: Lyu M 

PROVIDER: S-EPMC9161592 | biostudies-literature | 2022 Jun

REPOSITORIES: biostudies-literature