Arginase-1 Is Required for Macrophage-Mediated Renal Tubule Regeneration.

ABSTRACT:

Background

After kidney injury, macrophages transition from initial proinflammatory activation to a proreparative phenotype characterized by expression of arginase-1 (Arg1), mannose receptor 1 (Mrc1), and macrophage scavenger receptor 1 (Msr1). The mechanism by which these alternatively activated macrophages promote repair is unknown.

Methods

We characterized the macrophage and renal responses after ischemia-reperfusion injury with contralateral nephrectomy in LysM-Cre;Arg1^fl/fl mice and littermate controls and used in vitro coculture of macrophages and tubular cells to determine how macrophage-expressed arginase-1 promotes kidney repair.

Results

After ischemia-reperfusion injury with contralateral nephrectomy, Arg1

SUBMITTER: Shin NS 

PROVIDER: S-EPMC9161787 | biostudies-literature | 2022 Jun

REPOSITORIES: biostudies-literature