Wnt10b-overexpressing umbilical cord mesenchymal stem cells promote fracture healing via accelerated cartilage callus to bone remodeling.

ABSTRACT: The aim of this study was to investigate whether HUCMSCs^Wnt10b could promote long bone fracture healing. Commercially-available HUCMSCs^Emp (human umbilical cord mesenchymal stem cells transfected with empty vector) in hydrogel, HUCMSCs^Wnt10b in hydrogel and HUCMSCs^Wnt10b with the Wnt signaling pathway inhibitor IWR-1 were transplanted into the fracture site in a rat model of femoral fracture. We found that transplantation of HUCMSCs^Wnt10b significantly accelerated bone healing in a rat model of femoral fracture. Meanwhile, three-point bending test proved that the mechanical properties of the bone at the fracture site in the HUCMSC^Wnt10b treatment group were significantly better than those of the other treatment groups. To un

SUBMITTER: Hu Y 

PROVIDER: S-EPMC9161882 | biostudies-literature | 2022 Apr

REPOSITORIES: biostudies-literature