Efficient Assay and Marker Significance of NAD⁺ in Human Blood.

ABSTRACT: Oxidized nicotinamide adenine dinucleotide (NAD⁺) is a biological molecule of systemic importance. Essential role of NAD⁺ in cellular metabolism relies on the substrate action in various redox reactions and cellular signaling. This work introduces an efficient enzymatic assay of NAD⁺ content in human blood using recombinant formate dehydrogenase (FDH, EC 1.2.1.2), and demonstrates its diagnostic potential, comparing NAD⁺ content in the whole blood of control subjects and patients with cardiac or neurological pathologies. In the control group (n = 22, 25-70 years old), our quantification of the blood concentration of NAD⁺ (18 μM, minimum 15, max 23) corresponds well to NAD⁺ quantifications reported in literature. In pa

SUBMITTER: Balashova NV 

PROVIDER: S-EPMC9162244 | biostudies-literature | 2022

REPOSITORIES: biostudies-literature