Computational multifocus fluorescence microscopy for three-dimensional visualization of multicellular tumor spheroids.

ABSTRACT:

Significance

Three-dimensional (3D) visualization of multicellular tumor spheroids (MCTS) in fluorescence microscopy can rapidly provide qualitative morphological information about the architecture of these cellular aggregates, which can recapitulate key aspects of their in vivo counterpart.

Aim

The present work is aimed at overcoming the shallow depth-of-field (DoF) limitation in fluorescence microscopy while achieving 3D visualization of thick biological samples under study.

Approach

A custom-built fluorescence microscope with an electrically focus-tunable lens was developed to optically sweep in-depth the structure of MCTS. Acquired multifocus stacks were combined by means of postprocessing algorithms performed in the Fourier domain.

Results

Images with rele

SUBMITTER: Alonso JR 

PROVIDER: S-EPMC9162503 | biostudies-literature | 2022 Jun

REPOSITORIES: biostudies-literature