R-based method for quantitative analysis of biofilm thickness by using confocal laser scanning microscopy.

ABSTRACT: Microscopy is mostly the method of choice to analyse biofilms. Due to the high local heterogeneity of biofilms, single and punctual analyses only give an incomplete insight into the local distribution of biofilms. In order to retrieve statistically significant results a quantitative method for biofilm thickness measurements was developed based on confocal laser scanning microscopy and the programming language R. The R-script allows the analysis of large image volumes with little hands-on work and outputs statistical information on homogeneity of surface coverage and overall biofilm thickness. The applicability of the script was shown in microbial fuel cell experiments. It was found that Geobacter sulfurreducens responds differently to poised anodes of different material so that the

SUBMITTER: Fruhauf HM 

PROVIDER: S-EPMC9162930 | biostudies-literature | 2022 Jun

REPOSITORIES: biostudies-literature