A single allele of the hsa-miR-302/367 cluster maintains human pluripotent stem cells.

ABSTRACT:

Introduction

In a diploid organism, two alleles from a single genetic locus are expressed to generate a normal phenotype. Heterozygous deleterious mutation causes a reduction of functional proteins to a half dose and insufficient amounts of functional proteins can occur to generate an in-normal phenotype, namely haploinsufficiency. Heterozygous deleterious mutation of microRNAs (miRs), non-coding RNAs that regulate the expression level of target transcripts, is still not well understood. The hsa-miR-302/367 cluster is the most abundant and specifically up-regulated miR cluster in human embryonic stem cells (ESCs) and induced pluripotent stem cells (iPSCs) and plays an important role in the maintenance of pluripotency.

Methods

We targeted the hsa-miR-302/367 region via a Cas9

SUBMITTER: Sugawara T 

PROVIDER: S-EPMC9162946 | biostudies-literature | 2022 Dec

REPOSITORIES: biostudies-literature