High-speed optical resolution photoacoustic microscopy with MEMS scanner using a novel and simple distortion correction method.

ABSTRACT: Optical resolution photoacoustic microscopy (OR-PAM) is a remarkable biomedical imaging technique that can selectively visualize microtissues with optical-dependent high resolution. However, traditional OR-PAM using mechanical stages provides slow imaging speed, making it difficult to biologically interpret in vivo tissue. In this study, we developed a high-speed OR-PAM using a recently commercialized MEMS mirror. This system (MEMS-OR-PAM) consists of a 1-axis MEMS mirror and a mechanical stage. Furthermore, this study proposes a novel calibration method that quickly removes the spatial distortion caused by fast MEMS scanning. The proposed calibration method can easily correct distortions caused by both the scan geometry of the MEMS mirror and its nonlinear motion by running an image seque

SUBMITTER: Shintate R 

PROVIDER: S-EPMC9163157 | biostudies-literature | 2022 Jun

REPOSITORIES: biostudies-literature