Microvascular imaging of the unstained human superior colliculus using synchrotron-radiation phase-contrast microtomography.

ABSTRACT: Characterizing the microvasculature of the human brain is critical to advance understanding of brain vascular function. Most methods rely on tissue staining and microscopy in two-dimensions, which pose several challenges to visualize the three-dimensional structure of microvessels. In this study, we used an edge-based segmentation method to extract the 3D vasculature from synchrotron radiation phase-contrast microtomography (PC-μCT) of two unstained, paraffin-embedded midbrain region of the human brain stem. Vascular structures identified in PC-μCT were validated with histology of the same specimen. Using the Deriche-Canny edge detector that was sensitive to the boundary between tissue and vascular space, we could segment the vessels independent of signal variations in PC-μCT images. From

SUBMITTER: Lee JY 

PROVIDER: S-EPMC9163179 | biostudies-literature | 2022 Jun

REPOSITORIES: biostudies-literature