Development of ONT-cappable-seq to unravel the transcriptional landscape of Pseudomonas phages.

ABSTRACT: RNA sequencing has become the method of choice to study the transcriptional landscape of phage-infected bacteria. However, short-read RNA sequencing approaches generally fail to capture the primary 5' and 3' boundaries of transcripts, confounding the discovery of key transcription initiation and termination events as well as operon architectures. Yet, the elucidation of these elements is crucial for the understanding of the strategy of transcription regulation during the infection process, which is currently lacking beyond a handful of model phages. We developed ONT-cappable-seq, a specialized long-read RNA sequencing technique that allows end-to-end sequencing of primary prokaryotic transcripts using the Nanopore sequencing platform. We applied ONT-cappable-seq to study transcription of <

SUBMITTER: Putzeys L 

PROVIDER: S-EPMC9163698 | biostudies-literature | 2022

REPOSITORIES: biostudies-literature