The long β2,3-sheets encoded by redundant sequences play an integral role in the channel function of P2X7 receptors.

ABSTRACT: P2X receptors are a class of nonselective cation channels widely distributed in the immune and nervous systems, and their dysfunction is a significant cause of tumors, inflammation, leukemia, and immune diseases. P2X7 is a unique member of the P2X receptor family with many properties that differ from other subtypes in terms of primary sequence, the architecture of N- and C-terminals, and channel function. Here, we suggest that the observed lengthened β2- and β3-sheets and their linker (loop β2,3), encoded by redundant sequences, play an indispensable role in the activation of the P2X7 receptor. We show that deletion of this longer structural element leads to the loss of P2X7 function. Furthermore, by combining mutagenesis, chimera construction, surface expression, and protein stability ana

SUBMITTER: Ma XF 

PROVIDER: S-EPMC9163701 | biostudies-literature | 2022 Jun

REPOSITORIES: biostudies-literature