Laminin 511-E8 Fragment Offers Superior Adhesion Properties for Gastric Cancer Cells Compared with Full-Length Laminin 511.

ABSTRACT:

Background

The interaction between cancer cells and laminin (Ln) is a key event in tumor invasion and metastasis. Previously, we determined the effect of full-length Ln511 on gastric cancer cells. However, the interactions between the Ln511-E8 fragment, a truncated protein of Ln511, and gastric cancer cells have not been investigated.

Methods

We investigated the adhesion properties of gastric cancer cells to full-length Ln511 and Ln511-E8 fragments.

Results

The proliferation of four gastric cancer cell lines (SH-10-TC, MKN74, SC-6-JCK, and MKN45) was highest on the Ln511-E8 fragment. Further, a larger cytoplasm was observed in SH-10-TC and MKN74 cells cultured on full-length Ln511 or Ln511-E8 fragments. The percentage of adhesive cells was highest on the Ln511-E8 frag

SUBMITTER: Iwamuro M 

PROVIDER: S-EPMC9164087 | biostudies-literature | 2022 Apr

REPOSITORIES: biostudies-literature