Brain proteome profiling implicates the complement and coagulation cascade in multiple system atrophy brain pathology.

ABSTRACT:

Background

Multiple system atrophy (MSA) is a rare, progressive, neurodegenerative disorder presenting glia pathology. Still, disease etiology and pathophysiology are unknown, but neuro-inflammation and vascular disruption may be contributing factors to the disease progression. Here, we performed an ex vivo deep proteome profiling of the prefrontal cortex of MSA patients to reveal disease-relevant molecular neuropathological processes. Observations were validated in plasma and cerebrospinal fluid (CSF) of novel cross-sectional patient cohorts.

Methods

Brains from 45 MSA patients and 30 normal controls (CTRLs) were included. Brain samples were homogenized and trypsinized for peptide formation and analyzed by high-performance liquid chromatography tandem mass spectrometry (LC-

SUBMITTER: Rydbirk R 

PROVIDER: S-EPMC9164190 | biostudies-literature | 2022 Jun

REPOSITORIES: biostudies-literature